Changsha Staherb Natural Ingredients Co.,Ltd.
Determination of Magnolol and Honokiol by HPLC
a. Nanopure water b. Methanol (HPLC)
HPLC (Shimadzu) Analytical balance (1/10000)
Ultrasonic cleaner 0.45μm PTFE filter
50ml volumetric flask Screw cap vials
3) HPLC condition
Column: Kromasil C18 5m 4.6×200mm
Detector: UV 294nm
Mobile Phase: A= water B= MeOH
Flow Rate: 1.0ml/min
Accurately weigh 30mg extraction into 50ml volumetric flask. Add MeOH to
volume and sonicate to mix, filter through a 0.45μm PTFE filter into a screw cap vial
and proceed with the analysis.
Standard preparation /calibration
Prepare a standard solution (approximately 0.2mg/ml in MeOH) using a standard of
Magnolol，Sonicate for about 10 minutes and transfer to screw-cap vials and can be
stored in the refrigeration.
Determine the peak areas of the Maglolol on the sample chromatogram and compare
to the corresponding peaks in the chromatogram for the standard solution. Calculate
the weight percent.