Macleaya cordata extract HPLC
Column：Hyper ODS C18
Mobile Phase: AcCN:H2O =40:60
Column diameter: 4.6mm
Pressure: 8.5 Mpa
Column temperature: room temperature
Injection volume: 5μl
Standard preparation /calibration
Accurately weigh 5mg Sanguinarine Reference Substance solution into 50ml Brown bottle. Add mobile phase to the scale, shake it well. Take 2ml of precise measurement and put it in 25 ml brown bottle, add methanol to the scale,shake it well, then abtain a susbstance solution.
Accurately weigh appropriate amount of Macleaya cordata extract sample, into 50 ml Brown volumetric flask,Add 30ml mobile phase and Ultrasound oscillation for about 30 minutes.Static to room temperature compatibility to mobile phase,filter through a 0.45μm PTFE filter then get the obtain a sample solution.
3. Determination method
Precise absorption respectively of reference solution and sample solution for 5ml each,Injection liquid chromatography and determination it ,and then it can be obtained.