MOA of Amygdalin
HPLC chromatographic conditions are carried out as following: column is packed with the octadecyl silane chemically bonded silica, flowing phase is composed with methanol and water (20:80). detection wavelength:210nm;flow rate:1.0ml/min.
1) Precisely measure the product 0.3g, put it into conical flask , add 50 ml 70% methanol, accurately weighed, supersonic treatment (power 250W, frequency 35kHz), cool it. Weight again. Using 70% methanol to make up the lost. Shake up. Filtering. Measuring off 5ml filtrate. Add it into 25ml brown glass flask volumetric. Add methanol to index mark. Shake up. That is test solution.
- taking amygdalin reference samples, weigh precisely, add 70% ethanol to make 80ug/ml solution
3)Injection standard substance solution and sample solution each 10 μ l, in the provision of chromatographic conditions, chromatographic analysis and record chromatograph chart, according to the external standard method to peak area computation.