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藤茶提取物二氢杨梅素 Dihydromyricetin
杨梅素 Myricetin
木犀草素Luteolin
苦杏仁甙amygdalin
绿原酸Chlorogenic acid
芹菜素apigenin
厚朴提取物Magnolia bark extract
厚朴酚magnolol
和厚朴酚Honokiol
厚朴总酚magnolol+honokiol
博落回提取物Macleaya cordata extract
血根碱sanguinarine
白屈菜红碱Chelerythrine
迷迭香提取物rosemary leaf extract
迷迭香酸rosmarnic aicd
鼠尾草酸Carnosic acid
熊果酸Ursolic acid
鼠尾草酸油carnosic acid liquid oil
绿咖啡豆提取物green coffee bean extract
金银花提取物honeysuckle flower extract
巴拿巴叶提取物Banaba leaf extract
枇杷叶提取物Loquat leaf extract
漆黄素Fisetin
虎杖甙Polydatin
芒果甙mangiferin
白桦脂醇Betulin
石杉碱甲huperzine a
阿魏酸Ferulic acid
白藜芦醇Resveratrol

苦杏仁甙amygdalin 

苦杏仁提取物苦杏仁甙

来源: 苦杏仁籽

有效成分: 苦杏仁甙

拉丁名: Semen Armeniacae Amarum

规格: 1%-99%

检测方式: HPLC UV

级别: 食品级别

外观: 白色精细粉末

提取方式: 溶剂提取

苦杏仁甙简介

杏仁甙主要存在于苦杏、苦扁桃、桃、油桃(nectarines)、枇杷、李子、苹果、黑樱桃等果仁和叶子中,苦杏仁皮中不含苦杏仁甙。苦杏仁甙由龙胆二糖和苦杏仁睛组成的β-型糖苷,分子式:C20H27NO11,分子量:457.43。其三水合物为斜方柱状结晶,熔点200℃,无水物熔点约有20℃,[α]:20D-42℃。1g可溶于12mlH2O,900ml乙醇及11ml乙醇,易溶于沸水。

苦杏仁,味苦、微温、归肺、大肠经。富含蛋白质、糖类、胡萝卜素、脂肪、维生素B族、维生素C、还含有苦杏仁甙、脂肪油、膳食纤维、钙元素,钾元素,镁元素及8种脂肪酸等等营养成分。具有止咳平喘、润肠通便的功效。苦杏仁中的苦杏仁苷有镇痛作用,还可以杀死癌细胞,延长癌症病人寿命;含有的胡萝卜素还有抑制肺癌、预防肿瘤的作用,能提高人体的免疫力;而且含有的脂肪油还能润便通肠、促进皮肤微循环,起到美容作用。
【产品名称】苦杏仁甙
【英文名称 】amygdalin
【产品来源】苦杏仁
【主要成分】苦杏仁甙
【提取规格】98%  99%
【产品性状】白色晶体粉末
【功效用途】:镇咳、平喘、镇痛

苦杏仁甙的功效:

1.镇咳、平喘, 抗炎、镇痛作用

2.对消化系统的作用 

3.抗肿瘤作用 ,能降血脂血糖

4.美容作用

 

苦杏仁甙的应用:

1.制药业

2.食品行业

3.护肤品行业

4.保健品行业

 

苦杏仁甙HPLC含量测定

HPLC法测定苦杏仁甙含量的操作规程

起草单位:长沙上禾生物科技有限公司

日期:

目  的:HPLC法测定苦杏仁甙含量的操作规程

责任人:QC员。

内  容:

1、 试剂

1.1乙腈(HPLC级)

1.2 蒸馏水(AR级)

1.3磷酸(HPLC级)

1.4 甲醇(HPLC级)

2、 对照品

苦杏仁甙(购自中国药品生物制品检定所)

3、 仪器和用具

3.1 电子天平(1/100000)

3.2 玻璃仪器:100ml容量瓶

3.3 安捷伦1220 HPLC

4、液相条件

4.1流动相:乙腈一0.1%磷酸溶液(8:92)

4.2检测波长:207nm

4.3流速:1ml/min

4.4柱温:30℃

5 、  操作步骤

5.1 对照品溶液的制备:在110℃干燥至恒重的苦杏仁苷对照品适量,精密称定,加甲醇制成每1m1含40μlg的溶液,即得

 5.2样品溶液制备  精密称取在110℃干燥至恒重的苦杏仁甙,精密称定,加甲醇制成每1m1含40μlg的溶液,即得

6、检测

取对照品溶液和样品溶液0.22μm针孔过滤器过滤,进样20µl,根据峰面积计算样品含量。

含量按以下公式计算:

           A2 x C1 x T

   R=------------------------- x 100

       A1 x C2

C1: 对照品的参考浓度,mg/ml

C2: 样品的参考浓度,mg/ml

A1: 对照品溶液的色谱主峰值面积

A2: 样品溶液的色谱主峰值面积

T:  对照品的含量

参考文献:

 

 1.Akahori, A. and Kagawa, K., Enzymatic hydrolysis of amygdalin in “Tōnin,” “Kyōnin” and a traditional preparation containing “Tōnin”. Shoyakugaku Zasshi, 37, 241–247 (1983).

2.Cairns, T., Froberg, J. E., Gonzales, S., Langham, W. S., Stamp, J. J., Howie, J. K., and Sawyer, D. T., Analytical chemistry of amygdalin. Anal. Chem., 50, 317–322 (1978).
3.Culliton, B. J. and Sloan-Kettering: The Trials of an Apricot Pit. Science, 182, 1000–1001 (1973).
4.Dybowski, R., Examination of amygdalin preparations. Anal. Proc., 18, 316–318(1978).
5.Fischer, E., Ueber ein neudes dem amygdalin ahnliches glucosid. Ber. Deut. Chem. Ges., 28, 1508–1511(1985).
6.Heisman, D. R. and Knight, D. J., The enzymic hydrolysis of amygdalin. Biochem. J., 103, 528–534(1967).
7.Hwang, E. Y., Lee, J. H., Lee, Y. M., and Hong, S. P., Reversephase HPLC separation of D-amygdalin and neoamygdalin and optimum conditions for inhibition of racemization of amygdalin. Chem. Pharm. Bull., 50, 1373–1375 (2002).
8.Isozaki, T., Matano, Y., Yamamoto, K., Kosaka, N., and Tani, T., Quantitative determination of amygdalin epimers by cyclodextrin-modified micellar electrokinetic chromatography. J. Chromatogr. A, 923, 249–254 (2001).
9.Koo, J. Y., Hwang, E. Y., Cho, S. H., Lee, J. H., Lee, Y. M., and Hong, S. P., Quantitative determination of amygdalin epimers from armeniacae semen by liquid chromatography. J. Chromatogr. B, 814, 69–73 (2005).
10.Smith, D. J. and Weber, J. D., Preparative and analytical separation of amygdalin and related compounds in injectables and tablets by reversed-phase HPLC and the effect of temperature on the separation. J. Chromatogr. Sci., 22, 94–98 (1984).
11.Stobaugh, J. E., Sternson, L. A., and Repta, A. J., Clinical method for analysis of amygdalin in human-plasma. Anal. Lett., 9, 753–764 (1978).
12.Takayama, Y. and Kawai, S., Improved gas chromatography of amygdalin and its diastereomer. J. Chromatogr., 197, 240–245 (1980).
13.Takayama, Y. and Kawai, S., Study on the prevention of racemization of amygdalin. Chem. Pharm. Bull., 32, 778–781(1984).
14.Xu, L. and Wang, W., Xing Ren (apricot kernel, semen pruni armeniacae). Chin. Mater. Med., 431–432 (2002). 
15.The Pharmacokinetics of Amygdalin
A. G. Rauws, M. Olling, A. Timmerman in Archives of Toxicology (1982)
16.Improvement of the extraction efficiency of d-amygdalin from Armeniacae Semen powder through inactivating emulsin and suppressing the epimerization of d-amygdalin
Armeniacae Semen contains not only amygdalin but also emulsin, which is an enzyme that hydrolyzes amygdalin. This hydrolysis reaction has been a major...d-amygdalin, the extraction time was kept to less...d-amygdalin
Ha-Jeong Kwon, Je-Hyun Lee, Seon-Pyo Hong in Archives of Pharmacal Research (2010)

17.THE AMYGDALIN (‘LAETRILE’) CLINICAL TRIAL BY THE NCI
InPharma (1981)

18.Apricot kernal extract (amygdalin): serious health risks
Reactions Weekly (2016)

19.Pharmacology of amygdalin (laetrile) in cancer patients
Plasma and urine concentrations of amygdalin, whole-blood concentrations of cyanide, and...2...) and oral (500-mg tablet) administration of amygdalin. To measure low plasma concentrations of amygdalin following o...

Matthew M. Ames, Thomas P. Moyer, John S. Kovach… in Cancer Chemotherapy and Pharmacology (1981)

20.Prevention of epimerization and quantitative determination of amygdalin in Armeniacae Semen with Schizandrae Fructus solution
Armeniacae Semen not only contains amygdalin, but emulsin also, which is an enzyme that hydrolyzes amygdalin. The extraction yield of amygdalin from Armeniacae Semen was low, due to ... solution was used as the e...

Woo-Sang Joo, Ji-Seon Jeong, Hyogeun Kim, Yong-Moon Lee… in Archives of Pharmacal Research (2006)

21.Application of a quantitative 1H-NMR method for the determination of amygdalin in Persicae semen, Armeniacae semen, and Mume fructus
A quantitative 1H-NMR method (qHNMR) was used to measure the amygdalin content of Persicae semen, Armeniacae semen, and Mume fructus, in each of which amygdalin constitutes a major component. The purity of amygdalin

Rie Tanaka, Akane Nitta, Akito Nagatsu in Journal of Natural Medicines (2014)

22.Simple screening procedure for microorganisms to degrade amygdalin
Amygdalin is a cyanogenic glycoside occurring among others ... screening microbial cultures for their ability to degrade amygdalin is a convenient micro-titre version based...

L. Brimer, G. Tunçel, M. J. R. Nout in Biotechnology Techniques (1993)

23.Pharmacokinetic Effects of Cinnamic Acid, Amygdalin, Glycyrrhizic Acid and Liquiritin on Ephedra Alkaloids in Rats
Ephedra alkaloids, including (EP), pseudo (PEP) and methyl (MEP), are sympathomimetic compounds with known toxicities but many Ephedra (Ephedrae herba...) preparations, such as Ephedra...

Yinghong Tang, Mengkai Zheng, Yu-Lin Chen… in European Journal of Drug Metabolism and Ph… (2017)

24.Cyanide formation in preparations from Chlorella vulgaris Beijerinck: Effect of sonication and amygdalin addition
A critical evaluation of a method for recovering HCN from cell extracts is presented. Since crude extracts often bind or metabolize HCN extensively, the HCN recovered by distillation at room temperature repres...

Hans-Siegfried Gewitz, Elfriede K. Pistorius, Helga Voss, Birgit Vennesland in Planta (1976)

25.In vitro and in silico anticancer activity of amygdalin on the SK-BR-3 human breast cancer cell line
In spite of several studies that have shown the cytotoxic effects of amygdalin on the different cancer cell lines, however, the chemopreventive potential of amygdalin on the breast cancer cell line is ... complet...

Bahman Moradipoodeh, Mostafa Jamalan, Majid Zeinali… in Molecular Biology Reports (2019)

26.Über ein neues, dem Amygdalin ähnliches Glucosid
Das Amygdalin, welches bekanntlich durch Emulsin im Sinne der... gespalten wird, ist schon vor 25 Jahren von Hrn. Hugo Schiff1) mit dem ...

Emil Fischer in Untersuchungen Über Kohlenhydrate und Fermente (1884–1908) (1909)

27.Sensitive Monitoring of Amygdalin and 5-Hydroxytryptamine in Food Supplements Using HILIC OH5 Chromatography
Amygdalin is a cyanogenic glycoside found in pits ... Recently, in some countries, food supplements containing amygdalin have been offered for the use in the alternative cancer treatment. The effectivity of amygdalin

Lucie Havlíková, Martina Parmová, Petr Chocholouš, Petr Solich in Food Analytical Methods (2016)

28.The anti-cancer effect of amygdalin on human cancer cell lines
Derived from rosaceous plant seed, amygdalin belongs to aromatic cyanogenic glycoside group, and ... In this study, we objected to investigate amygdalin effect on two antiapoptotic genes (Survivin, XIAP) and two ...

Asghar Arshi, Sayed Mostafa Hosseini… in Molecular Biology Reports (2019)

29.The pharmacokinetics of amygdalin
Amygdalin (d-mandelonitrile-β-d...-gentiobioside) is a cyanogenic glycoside claimed to show anti-cancer activity, sold under the incorrect name “Laetrile”. For a sensible discussion of its alleged activity and it...

A. G. Rauws, M. Olling, A. Timmerman in Archives of Toxicology (1982)

30.Synergistic and protective effect of atorvastatin and amygdalin against histopathological and biochemical alterations in Sprague-Dawley rats with experimental endometriosis
The aim of the present study was to evaluate the protective effects of combined atorvastatin and amygdalin in a rat model of endometriosis. Tumor ... suggested that combined treatment consisting of atorvastatin a...

Fang Hu, Yichuan Hu, Fangxin Peng in AMB Express (2019)

 


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