藤茶提取物二氫楊梅素 Dihydromyricetin

藤茶提取物二氫楊梅素

來源:藤茶

拉丁名稱:Ampelopsis grossedentata(Hand-Mazz)WT Wan

提取部位:葉子

規格: 5%-98%
有效成份 :二氫楊梅素

CAS:327-97-9

檢測方式:HPLC
顏色: 類白色粉末

編輯連結

植物介紹：

顯齒蛇葡萄( Ampelopsis grossedentata(Hand - Mazz)WT Wan ) 顯齒蛇葡藤,茶屬葡萄科，蛇葡萄屬，又稱藤茶，木質藤本，植物體全部無毛;卷須二分叉，長可達 12cm。葉為二回羽狀復葉，長可達 21cm，小葉9 ~15 片，最下羽片有小葉3 片;枝頂部葉為一回羽狀復葉;小葉草質或薄紙質，長橢圓形、卵形、菱狀狹卵形或披針形, 長 2.5 ~ 7cm，寬 13cm，頂端漸尖或急尖，基部楔形或寬楔形，邊緣有粗鋸齒，上面綠色, 下面淡綠色，幼葉略帶紫紅色;葉柄長 1.5 ~3cm，枝上部葉几無柄，頂生小葉具柄，側生小葉無柄，

稍偏斜。聚傘花序與葉對生;總花梗長 3~5cm;花小，黃綠色;花萼淺杯狀，花瓣 5片，長圓形;雄蕊 5 枚;花盤淺杯狀。漿果近球形，直徑 7~10mm。花期 6~9 月，果期 7~11 月。采收時間為 4~8 月份。

產地生源:湖南、雲南、廣西、廣東、江西、貴州、湖北、福建(南靖、上杭、龍岩、連城、長汀、永安、三明、沙縣、寧化、建寧、南平、建甌、建陽、武夷山、浦城、順昌、光澤)等海拔 400~1300 米的山地灌叢中、林中、石上、河邊。

化學成分: 主要含有二氫楊梅素（dihydromyricetin ),楊梅素( myricetin )、槲皮素(quercetin)、槲皮素,花旗松素( taxifolin)、洋芹苷(apiin)等黃酮類物質。其主要成分為二氫楊梅素。

二氫楊梅素的功效

二氫楊梅素為葡萄屬植物藤茶的提取物，是藤茶中的主要活性成分黃酮類化合物，此類物質具有清除自由基、抗氧化、抗血栓、抗腫瘤、消炎等多種奇特功效;而二氫楊梅素是較為特殊的一種黃酮類化合物，在解除醇中毒、預防酒精肝、脂肪肝、抑制肝細胞惡化、降低肝癌的發病率、抗高血壓、抑制體外血小板聚集和體內血栓的形成、降低血脂和血糖水平，提高 SOD 活性以及保肝護肝等方面具有特殊功效。

藤茶提取物 - 二氫楊梅素簡介

藤茶，是葡萄科蛇葡萄屬的一種野生木質藤本植物，提取成分為二氫楊梅素，其中主要活性成分為黃酮類化合物，此類物質具有清除自由基、抗氧化、抗血栓、抗腫瘤、消炎等多種奇特功效;而二氫楊梅素是較為特殊的一種黃酮類化合物，除具有黃酮類化合物的一般特性外，還具有解除醇中毒、預防酒精肝、脂肪肝、抑制肝細胞惡化、降低肝癌的發病率等作用。是保肝護肝，解酒醒酒的良品。

HPLC法測定二氫楊梅素含量的操作規程

起草單位：長沙上禾生物科技有限公司

目的：HPLC法測定二氫楊梅素含量的操作規程

責任人：QC員。

內容：

1、試劑

1.1 甲醇（HPLC級）

1.2 蒸餾水（AR級）

1.3冰醋酸（HPLC級）

2、對照品

二氫楊梅素（購自中國藥品生物制品檢定所）

3、儀器和用具

3.1 電子天平（1/100000）

3.2 玻璃儀器：100ml容量瓶

3.3 安捷倫1220 HPLC

4、液相條件

4.1流動相：甲醇：水：冰醋酸=50:50:1

4.2檢測波長：292nm

4.3流速：1ml/min

4.4柱溫：30℃

5 、操作步驟

5.1 對照品溶液的制備 精密稱取在110℃乾燥至恆重的二氫楊梅素對照品10mg，置50ml容量瓶中，加50%甲醇適量，超聲處理10min使溶解，放冷，加50%甲醇至刻度，搖勻，備用。

5.2樣品溶液制備 精密稱取在110℃乾燥至恆重的二氫楊梅素樣品10mg，置50ml容量瓶中，加50%甲醇適量，超聲處理10min使溶解，放冷，加50%甲醇至刻度，搖勻，備用。

6、檢測

取對照品溶液和樣品溶液0.22μm針孔過濾器過濾，進樣20µl，根據峰面積計算樣品含量。

含量按以下公式計算：

A2 x C1 x T

R=------------------------- x 100

A1 x C2

C1: 對照品的參考濃度，mg/ml

C2: 樣品的參考濃度，mg/ml

A1: 對照品溶液的色譜主峰值面積

A2: 樣品溶液的色譜主峰值面積

T: 對照品的含量

藤茶生產工藝流程

藤茶提取物-二氫楊梅素生產工藝流程：

原料→挑洗→去雜→粉碎→乙醇提取→濃縮→離心→上大孔樹脂柱→20%,40%,60%,80%乙醇分別洗脫

→分段收集→脫色→結晶→乾燥→混合、篩分、包裝→檢測合格后入庫

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