Determination of Mogroside by HPLC-ELSD 1) Reagents a. Nanopure water b. Acetonitrile (HPLC) 2) Apparatus HPLC (Shimadzu) Analytical balance (1/10000) Ultrasonic cleaner 0.45μm PTFE filter 50ml volumetric flask Screw cap vials ELSD detector(Alltech)
3) HPLC condition Column: Polaris C18 5μm , 4.6×250mm Mobile Phase: A:Water B: Acetonitrile Flow Rate: 1.0 ml/min Injection: 10μl Gradient: Time(min) %A %B 0.01 85 15 22 40 60 24 40 60 25 85 15 Drift tube temperature :108℃ Gas flow:2.8L/min Impactor: Off Gain:2
4) Procedure Samples Preparation Accurately weigh 30mg extraction into 10ml volumetric flask. Add Methanol to volume and sonicate to mix, filter through a 0.45μm PTFE filter into a screw cap vial and proceed with the analysis.
Standard preparation /calibration Prepare a standard solution (approximately 0.2mg/ml in MEOH) using a commercial reference standard of Mogroside ,Sonicate for about 10 minutes and transfer to screw-cap vials and can be stored in the refrigeration.
5) Calculation Determine the peak area’s logarithm of the Mogroside on the sample chromatogram and compare to the corresponding peaks in the chromatogram for the sample solution.
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