| Determination of Resvertrol by HPLC
a. Acetonitrile (HPLC) b. Nanopure water
HPLC (Shimadzu) Analytical balance (1/10000) Ultrasonic cleaner
0.45μm PTFE filter 50ml volumetric flask Screw cap vials
3) HPLC condition
Column: Shim-pack VP-ODS C18 5μm , 4.6×150mm
Detector: UV 302nm
Mobile Phase: A=Water B= Acetonitrile
A/B=70/30(isocratic) 20min stop
Flow Rate: 1.0ml/min
Accurately weigh 15mg extraction into 50ml volumetric flask. Add Methanol to volume and sonicate to mix, filter through a 0.45μm PTFE filter into a screw cap vial and proceed with the analysis.
Standard preparation /calibration
Prepare a standard solution (approximately 0.2mg/ml in water) using a commercial reference standard of Resvertrol，Sonicate for about 10 minutes and transfer to screw-cap vials and can be stored in the refrigeration.
Determine the peak areas of the Resvertrol on the sample chromatogram and compare to the corresponding peaks in the chromatogram for the reference standard solution. Calculate the weight percent.